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GCLC Antikörper (N-Term)

Name: GCLC Antikörper (N-Term)
SKU: ABIN655884
Price: 467.5 EUR
Availability: InStock

GCLC Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB31687 unconjugated

GCLC Antibody (N-term) (ABIN655884 and ABIN2845285) western blot analysis in Jurkat cell line lysates (35 μg/lane). (GCLC Antikörper (N-Term))

GCLC Antibody (N-term) (ABIN655884 and ABIN2845285) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). (GCLC Antikörper (N-Term))

FACS

Confocal immunofluorescent analysis of GCLC Antibody (N-term) (ABIN655884 and ABIN2845285) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GCLC Antikörper (N-Term))

GCLC Antibody (N-term) (ABIN655884 and ABIN2845285) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (GCLC Antikörper (N-Term))

IHC (p)

4 Abbildungen

(1 reference)

Produktnummer ABIN655884

Datenblatt (PDF)

Target Alle GCLC Antikörper anzeigen

GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
Bindungsspezifität
9

7

6

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-30, N-Term
Reaktivität
59

22

20

4

4

4

3

3

3

2

2

1

1

1

1

1

1

1
Human
Wirt
59

4
Kaninchen
Passende Sekundärantikörper
Klonalität
50

13
Polyklonal
Konjugat
37

4

3

3

2

2

2

2

2

2

2

2
Dieser GCLC Antikörper ist unkonjugiert
Applikation
45

26

22

12

10

9

9

5

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCLC.

Klon

RB31687

Isotyp

Ig Fraction

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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibody
GCLC Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Hund, Meerschweinchen, Pferd WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibody
GCLC Reaktivität: Human WB, IHC, ELISA, IF, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibody
GCLC Reaktivität: Human, Maus, Ratte, Schwein WB, IHC, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibody
GCLC Reaktivität: Human, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibody
GCLC Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibody
GCLC Reaktivität: Ratte WB, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibody
GCLC Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Hund, Meerschweinchen, Pferd, Saccharomyces cerevisiae WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibody
GCLC Reaktivität: Human WB, IF, IHC (p), FACS, EIA Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibody
GCLC Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Chen, Jiang, Hu, Zhang: "The protective role of resveratrol in the sodium arsenite-induced oxidative damage via modulation of intracellular GSH homeostasis." in: Biological trace element research, Vol. 155, Issue 1, pp. 119-31, (2014) (PubMed).
Target

GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung

GCLC (GCLC Produkte)

Hintergrund

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Molekulargewicht

72766

Gen-ID

2729

NCBI Accession

NP_001184044, NP_001489

UniProt

P48506

Pathways

Cell RedoxHomeostasis