SFPQ Antikörper (C-Term)

Produktnummer ABIN657824

Produktdetails anti-SFPQ Antikörper

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Bindungsspezifität: AA 676-705, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SFPQ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Homologie: M

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 676-705 amino acids from the C-terminal region of human SFPQ.

Klon: RB34049

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: SFPQ Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Haltbarkeit: 6 months

Details zu SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Andere Bezeichnung: SFPQ (SFPQ Produkte)

Synonyme: 1110004P21Rik antikoerper, 2810416M14Rik antikoerper, 5730453G22Rik antikoerper, 9030402K04Rik antikoerper, AU021830 antikoerper, D4Ertd314e antikoerper, Gm12940 antikoerper, OTTMUSG00000009329 antikoerper, PSF antikoerper, REP1 antikoerper, hm:zeh0027 antikoerper, wu:fa11h12 antikoerper, wu:fd10f03 antikoerper, zgc:85935 antikoerper, POMP100 antikoerper, splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antikoerper, splicing factor proline/glutamine-rich antikoerper, splicing factor proline and glutamine rich antikoerper, Sfpq antikoerper, sfpq antikoerper, SFPQ antikoerper

Hintergrund: DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

Molekulargewicht: 76149

Gen-ID: 6421

NCBI Accession: NP_005057

UniProt: P23246