ALK Antikörper (AA 200-335)

Produktnummer ABIN6654203

Produktdetails anti-ALK Antikörper

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Bindungsspezifität: AA 200-335

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALK Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Purified

Reinheit: Protein G affinity

Immunogen: A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.

Klon: ALK-1504

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant ALK antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ALK

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Andere Bezeichnung: ALK / Anaplastic Lymphoma Kinase (ALK Produkte)

Hintergrund: The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,  NPM-ALK, created by the t(2,5)(p23,q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5 % -10 % of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Pathways: RTK Signalweg