DMPO Antikörper

Produktnummer ABIN6655016

Hersteller Produkt- Nr.: 200-301-f21

Produktdetails

Target: DMPO

Reaktivität: Adenovirus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-301-f21

Hersteller: Rockland

Verwendungszweck: DMPO Antibody

Kreuzreaktivität (Details): DMPO is species independent.

Aufreinigung: Anti-DMPO Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Klon: N1664A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 25 μg

ELISA_Dilution: 1-10 μg/mL

IF_Microscopy_Dilution: 10 μg/mL

Kommentare:

Anti-DMPO Antibody tested by western blot, ELISA, IHC, IF microscopy and IP. Expect a band approximately ~90kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: DMPO

Substanzklasse: Chemical

Hintergrund:

Synonyms: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide

Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.