GFAP Antikörper

Produktnummer ABIN6655053

Hersteller Produkt- Nr.: 200-901-d60

Produktdetails anti-GFAP Antikörper

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-901-d60

Hersteller: Rockland

Verwendungszweck: Glial Fibrillary Acidic Protein (GFAP) Antibody

Kreuzreaktivität (Details): Anti-GFAP Antibody is directed against bovine GFAP.

Aufreinigung: The antibody is a total IgY fraction.

Immunogen: Anti-GFAP Antibody was produced by repeated immunizations with recombinant and extensively purified bovine GFAP.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:10000

Kommentare:

Suggested Applications: IHC
Anti-Glial Fibrillary Acidic Protein (Chicken) antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the GFAP proteins in the appropriate cell lysate or extract. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GFAP Antikörper (ABIN6655053)

Gleichman, Kawaguchi, Sofroniew, Carmichael: "A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences." in: Nature communications, Vol. 14, Issue 1, pp. 7426, (2023) (PubMed).

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Abstract: GFAP Produkte

Hintergrund:

Synonyms: gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345. Glial fibrillary acidic protein, GFAP antibody, neuroscience antibodies

Background: GFAP Antibody detects GFAP. Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers. Therefore, GFAP antibody is ideal for investigators involved in neuropathologic diseases and more generally in Neuroscience.

Gene Name: GFAP

Gen-ID: 281189

UniProt: Q28115