APP Antikörper

Produktnummer ABIN6655141

Hersteller Produkt- Nr.: 200-401-e87

Produktdetails anti-APP Antikörper

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-401-e87

Hersteller: Rockland

Verwendungszweck: Amyloid Fibrils (OC) Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Amyloid Fibrils (OC) from Human based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Amyloid Fibrils (OC) Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Amyloid Fibrils (OC) Antibody was produced from whole rabbit serum prepared by repeated immunizations with fibrils prepared from human Abeta42 synthetic peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Kommentare:

Suggested Applications: Other
Anti-Amyloid Fibrils (OC) Antibody is tested for use in IP, IF microscopy, IHC, and WB. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-APP Antikörper (ABIN6655141)

Lee, Park, Kim, Lee, Lee, Hwang, Lee, Lee, Yoon: "Plasmonic nanoparticle amyloid corona for screening Aβ oligomeric aggregate-degrading drugs." in: Nature communications, Vol. 12, Issue 1, pp. 639, (2021) (PubMed).

Nnah, Lee, Wessling-Resnick: "Iron potentiates microglial interleukin-1β secretion induced by amyloid-β." in: Journal of neurochemistry, Vol. 154, Issue 2, pp. 177-189, (2020) (PubMed).

Jeong, Kim, Chae, Lee, Yang, Kim, Kim, Lee, Lee, Choi, Yoon, Hwang: "Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40." in: Sensors (Basel, Switzerland), Vol. 18, Issue 6, (2018) (PubMed).

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: APP (APP Produkte)

Hintergrund:

Synonyms: Amyloid OC, Fibrils, Amyloid Oligomer αβ, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD1

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease.

Gene Name: APP

UniProt: P05067

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour