MBD1 Antikörper (N-Term)

Produktnummer ABIN6655183

Produktdetails anti-MBD1 Antikörper

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Aufreinigung: Anti-MBD1 Antibody was purified by affinity purification.

Immunogen:

Immunogen: Anti-MBD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminus of human MBD1.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-MBD1 Antibody is suitable for Chromatin Immunoprecipitation, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kDa in the appropriate cell lysate or extract.

ChIP Dilution: 1.5 μg per IP

ELISA Dilution: 1:1,000

Western Blot Dilution: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300/p>

Stabilizer: None

Konservierungsmittel: ProClin, Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Andere Bezeichnung: MBD1 (MBD1 Produkte)

Synonyme: CXXC3 antikoerper, PCM1 antikoerper, RFT antikoerper, Cxxc3 antikoerper, CCDC11 antikoerper, methyl-CpG binding domain protein 1 antikoerper, MBD1 antikoerper, Mbd1 antikoerper

Hintergrund:

Synonyms: Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, CXXC3, PCM1

Background: MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC's) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription. Anti-MBD1 Antibody is ideal for research in Epigenetics, Gene Expression and Chromatin Remodeling.

Gene Name: MBD1

Gen-ID: 4152

NCBI Accession: NP_001191065

UniProt: Q9UIS9