TLR7 Antikörper

Produktnummer ABIN6655379

Hersteller Produkt- Nr.: 200-301-i29

Produktdetails anti-TLR7 Antikörper

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Hersteller Produkt- Nr.: 200-301-i29

Hersteller: Rockland

Verwendungszweck: TLR7 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR7 from human based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-TLR7 Antibody was purified by Protein G chromatography.

Immunogen: TLR7 Antibody was produced in mice prepared by repeated immunizations with an internal portion of human TLR7 recombinant protein.

Klon: IMG4G6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Flow_Cytometry_Dilution: 1-2 μg/10^6 cells

Western_Blot_Dilution: 10 μg/mL

Kommentare:

Anti-TLR7 Antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, and IHC. Expect a band approximately 121kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TLR7

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Andere Bezeichnung: TLR7 (TLR7 Produkte)

Hintergrund:

Synonyms: Toll-like receptor 7

Background: Anti-TLR7 Antibody detects human TLR7. TLR7 is a 1049 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. It plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). It is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. It acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Anti-TLR7 Antibody is ideal for investigators that are involved in cytokin and grow factor research.

Gene Name: TLR7

Gen-ID: 51284

NCBI Accession: NP_057646

UniProt: Q9NYK1

Pathways: TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades