IKK alpha Antikörper

Produktnummer ABIN6655526

Hersteller Produkt- Nr.: 200-301-h82

Produktdetails anti-IKK alpha Antikörper

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IKK alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)

Hersteller Produkt- Nr.: 200-301-h82

Hersteller: Rockland

Verwendungszweck: IKK alpha Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with IKKα from Human, Mouse and New World Monkey based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-IKKα Antibody was purified by Protein G chromatography.

Immunogen: IKK alpha Antibody was produced in mice prepared by repeated immunizations by a His-tagged human IKK alpha protein at full-length.

Klon: 14A231

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1-2 μg/mL

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.25-0.5 μg/10^6 cells

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-IKK alpha antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 85-90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Andere Bezeichnung: IKK alpha (CHUK Produkte)

Hintergrund:

Synonyms: IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, 2.7.11.10, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16

Background: Anti-IKK alpha antibody detects human IKK alpha. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns. Anti-IKK alpha antibody is ideal for investigators involved in cytokines and growth factor and NFkappaB research.

Gene Name: CHUK

Gen-ID: 1147

NCBI Accession: NP_001269

UniProt: O15111

Pathways: PI3K-Akt Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine