MED23 Antikörper (AA 897-916)

Produktnummer ABIN6655801

Produktdetails anti-MED23 Antikörper

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Bindungsspezifität: AA 897-916

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED23 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Maus

Aufreinigung: This antiserum was processed by delipidation and defibrination and is directed against mouse DRIP130.  This reagent can be used to determine the presence of DRIP130 various immunological assays. Cross-reactivity occurs with human DRIP-130.  Reactivity with DRIP-130 isolated from other species is unknown.

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues  897-916 (based on human DRIP-130 residue numbering)  crosslinked to KLH.

Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for applications should be determined by the researcher. Rockland Immunochemical's anti-DRIP130 has been used to detect DRIP130 using nuclear extracts from mouse thymus, spleen, and brain. Unfortunately, we have not tested reactivity on any cell line extracts. The following standard procedure is recommended: separate 10 micrograms of thymus nuclear extract by 7.5 % SDS-PAGE (0.75 to 1 mm thick). Transfer to nitrocellulose without SDS in the transfer buffer (pre-equilibrate the gel in transfer buffer for 30 min prior to transfer). Blocking is performed with 5 % non-fat dry milk in TTBS for 1 hr at room temperature. Incubate anti-DRIP130 antibody at a 1:1,000 for 1 hr at room temperature. Dilute Donkey anti-Rabbit IgG-HRP 1:5,000 and react 1 hr at room temperature. A predominant band at ~130 KDa is detected under these conditions. Control or normal rabbit serum fails to recognize the 130 kDa protein.

Immunoprecipitation Dilution: 1:100 - 1:200

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.1 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Referenzen für anti-MED23 Antikörper (ABIN6655801)

Kasper, Fukuyama, Brindle: "T-cells null for the MED23 subunit of mediator express decreased levels of KLF2 and inefficiently populate the peripheral lymphoid organs." in: PLoS ONE, Vol. 9, Issue 7, pp. e102076, (2015) (PubMed).

Details zu MED23

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Andere Bezeichnung: DRIP130 (MED23 Produkte)

Synonyme: CG3695 antikoerper, Dmel\\CG3695 antikoerper, Gal11/SUR2 antikoerper, Med23 antikoerper, Trap150 antikoerper, Trap150beta antikoerper, dSUR2 antikoerper, dTRAP150beta antikoerper, CRSP3 antikoerper, MED23 antikoerper, ARC130 antikoerper, CRSP130 antikoerper, CRSP133 antikoerper, DRIP130 antikoerper, MRT18 antikoerper, SUR-2 antikoerper, SUR2 antikoerper, 130kDa antikoerper, 3000002A17Rik antikoerper, Crsp3 antikoerper, ESTM7 antikoerper, Sur2 antikoerper, X83317 antikoerper, mKIAA1216 antikoerper, crsp3 antikoerper, si:ch211-272f15.2 antikoerper, zgc:91979 antikoerper, Mediator complex subunit 23 antikoerper, mediator complex subunit 23 antikoerper, mediator of RNA polymerase II transcription subunit 23 antikoerper, mediator complex subunit 23 L homeolog antikoerper, Mediator of RNA polymerase II transcription subunit 23 antikoerper, MED23 antikoerper, med23 antikoerper, LOC412332 antikoerper, Med23 antikoerper, med23.L antikoerper, sur-2 antikoerper

Hintergrund:

Synonyms: rabbit anti-DRIP130antibody, Mediator of RNA polymerase II transcription subunit 23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2

Background: This antiserum recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130), and was tested for use in immunoprecipitation and immunoblotting.  DRIP-130 has been described elsewhere as Sur2, CRSP130, ARC130, NAT140, and TRAP150b.    DRIP-130 is component of a multi-protein complex termed DRIP that interacts with the vitamin D receptor (VDR), together, the DRIP proteins constitute a new cofactor complex.  DRIP binds to several nuclear receptors and mediates ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of two other cofactor complexes called ARC and TRAP, which are recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates.  Several DRIP/ARC/TRAP subunits are also components of other potentially related cofactors, such as CRSP, NAT, SMCC and the mouse Mediator, indicating that unique classes of activators may share common sets or subsets of cofactors. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.

Gene Name: MED23, DRIP130

Gen-ID: 9439, 28558074

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha