HSP70 1A Antikörper (AA 436-503)

Produktnummer ABIN6656050

Hersteller Produkt- Nr.: 200-301-a27

Produktdetails anti-HSP70 1A Antikörper

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Bindungsspezifität: AA 436-503

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70 1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-301-a27

Hersteller: Rockland

Verwendungszweck: Hsp70 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.

Aufreinigung: This Protein G purified monoclonal antibody reacts with human and mouse Hsp70 protein.

Sterilität: Sterile filtered

Immunogen: Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.

Klon: C92F3A-5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 10 μg/mL

IF_Microscopy_Dilution: 1:200-1:1000

Western_Blot_Dilution: 1:500-1:2000

Other: User Optimized

Kommentare:

Suggested Applications: Other
This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract.  In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 μg of heat shocked HeLa cell lysate by western blotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Hsp70 antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-HSP70 1A Antikörper (ABIN6656050)

Satoh, Stalder, McKercher, Williamson, Roth, Lipton: "Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential." in: ASN neuro, Vol. 7, Issue 4, (2016) (PubMed).

Ristic, Tsou, Guzi, Kanack, Scaglione, Todi: "USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila." in: The Journal of biological chemistry, Vol. 291, Issue 17, pp. 9161-72, (2016) (PubMed).

Satoh, Rezaie, Seki, Sunico, Tabuchi, Kitagawa, Yanagitai, Senzaki, Kosegawa, Taira, McKercher, Hoffman, Roth, Lipton: "Dual neuroprotective pathways of a pro-electrophilic compound via HSF-1-activated heat-shock proteins and Nrf2-activated phase 2 antioxidant response enzymes." in: Journal of neurochemistry, Vol. 119, Issue 3, pp. 569-78, (2011) (PubMed).

Details zu HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Andere Bezeichnung: HSPA1A (HSPA1A Produkte)

Hintergrund:

Synonyms: Heat shock 70 kDa protein 1 antibody, heat shock 70 kDa protein 1A antibody, Heat shock 70 kDa protein 1B antibody, Heat shock induced protein antibody, heat shock protein 70 antibody, HSP70 1 antibody

Background: Anti-Hsp 70 antibody is routinely used in cancer and signal transduction research. Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

Gene Name: HSPA1A

Gen-ID: 3304

NCBI Accession: NP_005336

UniProt: P0DMV8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process