Synaptobrevin (VAMP) Antikörper

Produktnummer ABIN6656054

Produktdetails

Target: Synaptobrevin (VAMP)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität: Rind (Kuh), Hamster, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)

Aufreinigung: Anti-Synaptobrevin (VAMP) Antibody is directed against human synaptobrevin. Anti-Synaptobrevin IgM was purified by chromatography. Reactivity is expected against bovine, mouse, pig, rat, rabbit and hamster.

Immunogen:

Immunogen: Anti-Synaptobrevin (VAMP) Monoclonal Antibody was produced in mouse by repeated immunizations with synaptic immunoprecipitate (crude) from human brain.

Immunogen Type: Native Protein

Klon: SP10

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Synaptobrevin antibody is suitable for use in Western Blotting and IHC. Expect a band of approximately 16 kDa in size corresponding to the VAMP proteins in Western blot of rat brain lysate. Anti-Synaptobrevin antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Antigendetails

Target: Synaptobrevin (VAMP)

Abstract: VAMP Produkte

Synonyme: 21.15 antikoerper, CG12210 antikoerper, Dmel\\CG12210 antikoerper, J antikoerper, SYB antikoerper, VAMP antikoerper, VAMP2 antikoerper, Vamp antikoerper, c-Syb antikoerper, l(2)46CFq antikoerper, l(2)46El antikoerper, syb antikoerper, LOC733071 antikoerper, Synaptobrevin antikoerper, synaptobrevin antikoerper, Syb antikoerper, LOC733071 antikoerper, Smp_006720.2 antikoerper, LOC100533331 antikoerper

Hintergrund:

Synonyms: Vesicle-associated membrane protein 1

Background: Synaptobrevin (also VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles. Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease.

Gene Name: VAMP1/2

Gen-ID: 6843

UniProt: P23763

Pathways: Tube Formation, Synaptic Vesicle Exocytosis