Bcl-2 Antikörper (AA 62-76)

Produktnummer ABIN6656060

Hersteller Produkt- Nr.: 200-401-222

Produktdetails anti-Bcl-2 Antikörper

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Bindungsspezifität: AA 62-76

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-401-222

Hersteller: Rockland

Verwendungszweck: BCL2 Antibody

Kreuzreaktivität (Details): The antibody is directed against human Bcl-2 and is useful in determining its presence in various assays.

Aufreinigung: This purified IgG antibody was prepared from monospecific rabbit antiserum by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: This purified IgG antibody was prepared from rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 62-76 (RDPVARTSPLQTPAA) of human Bcl-2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:500- 1:2,000

Other: User Optimized

Kommentare:

This purified IgG antibody against Human Bcl-2 has been tested for use in ELISA and immunoblotting.  The antibody recognizes a 27 kDa band corresponding to human bcl-2.  Anti-Bcl-2 shows perinuclear staining of Bcl-2.  Reactivity in other immunoassays is unknown.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Bcl-2 Antikörper (ABIN6656060)

Ramaswamy, Forbes, Minuesa, Gindin, Brown, Kharas, Krivtsov, Armstrong, Still, de Stanchina, Knoechel, Koche, Kentsis: "Peptidomimetic blockade of MYB in acute myeloid leukemia." in: Nature communications, Vol. 9, Issue 1, pp. 110, (2018) (PubMed).

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: Bcl-2 (BCL2 Produkte)

Hintergrund:

Synonyms: rabbit anti-Bcl-2 antibody, Apoptosis regulator Bcl 2 antibody, Apoptosis regulator Bcl2 antibody, AW986256 antibody, B cell CLL/lymphoma 2 antibody, B cell leukemia/lymphoma 2 antibody, B cell lymphoma 2 antibody, Bcl 2 antibody

Background: Bcl-2 is a human proto-oncogene located chromosome 18.  Its product is an integral membrane protein (also called Bcl-2) located in the membranes of the endoplasmic reticulum (ER), nuclear envelope, and in the outer membranes of the mitochondria.  The gene was discovered as the translocated locus in a B-cell leukemia (hence the name). This translocation is also found in some B-cell lymphomas.  In the cancerous B cells, the portion of chromosome 18 containing the BCL-2 locus has undergone a reciprocal translocation with the portion of chromosome 14 containing the antibody heavy chain locus. This t(14,18) translocation places the BCL-2 gene close to the heavy chain gene enhancer.  This enhancer is very active in B cells and therefore results in high levels of Bcl-2 expression in these cells.  High levels of the Bcl-2 protein protect the cells from early death by apoptosis. The Bcl-2 protein suppresses apoptosis by preventing the activation of the caspases that carry out the process.  It is conceived that introduction of the Bcl-2 gene into the cells of injured tissue will reduce cell death and improve the clinical outcome of the injury. Anti-BCL-2 Antibody is useful for researchers interested in lymphoma, breast cancer, and apoptosis research.

Gene Name: BCL2

Gen-ID: 596

NCBI Accession: NP_000624

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling