GFAP R416WT Antikörper (AA 411-422) (Biotin)
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- Target Alle GFAP R416WT Produkte
- GFAP R416WT
- Bindungsspezifität
- AA 411-422
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser GFAP R416WT Antikörper ist konjugiert mit Biotin
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Applikation
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA
- Kreuzreaktivität
- Human, Maus, Ratte (Rattus)
- Aufreinigung
- Anti-GFAP R416WT Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100% homology with the immunizing sequence. No cross-reactivity with other GFAP mutant proteins can be expected.
- Immunogen
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Immunogen: Anti-GFAP R416WT Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.
Immunogen Type: Peptide
- Klon
- S206B-9
- Isotyp
- IgG1
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- Applikationshinweise
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-GFAP R416WT Biotin Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1:1000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
0.1 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- GFAP R416WT
- Abstract
- GFAP R416WT Produkte
- Hintergrund
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Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody
Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.
Gene Name: GFAP
- Gen-ID
- 2670
- UniProt
- P14136
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