TLR5 Antikörper

Produktnummer ABIN6656728

Produktdetails anti-TLR5 Antikörper

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Anti-TLR5 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR5 from human, rat and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR5 from other sources has not been determined.

Immunogen:

Immunogen: TLR5 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a KLH-conjugated peptide corresponding to internal amino acids on human TLR5 protein.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 10 μg/mL (paraffin)

Application Note: Anti-TLR5 Antibody is suitable for use in Flow (I), IHC (p) and WB. Expect a band approximately 97 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 1-2 μg/10^6 cells

Western Blot Dilution: 1-3 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Andere Bezeichnung: TLR5 (TLR5 Produkte)

Synonyme: SLEB1 antikoerper, TIL3 antikoerper, TLR-5 antikoerper, sleb1 antikoerper, til3 antikoerper, tlr-5 antikoerper, tlr5 antikoerper, toll like receptor 5 antikoerper, toll-like receptor 5 antikoerper, toll like receptor 5 L homeolog antikoerper, toll-like receptor 5b antikoerper, TLR5 antikoerper, Tlr5 antikoerper, tlr5.L antikoerper, tlr-5 antikoerper, tlr5b antikoerper

Hintergrund:

Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Background: Anti-TLR5 Antibody detects human TLR5. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Anti-TLR5 Antibody is ideal for investigator involved in cytokine and growth factor research.

Gene Name: TLR5

Gen-ID: 7100

NCBI Accession: NP_003259

UniProt: O60602

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades