Aquaporin 1 Antikörper (N-Term)

Produktnummer ABIN6656923

Hersteller Produkt- Nr.: 600-401-e89

Produktdetails anti-Aquaporin 1 Antikörper

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Bindungsspezifität: N-Term

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aquaporin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-e89

Hersteller: Rockland

Verwendungszweck: Aquaporin 1 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Aquaporin 1 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Aquaporin 1 Antibody was purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: Aquaporin 1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of rat aquaporin 1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:200-400

Western_Blot_Dilution: 1:1000-4000

Kommentare:

Anti-Aquaporin 1 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~28.5kDa on specific lysates. May detect larger glycosylated bands ~35-50kDa. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Andere Bezeichnung: Aquaporin 1 (AQP1 Produkte)

Hintergrund:

Synonyms: AQP1, Water channel protein CHIP29, Aquaporin-CHIP, Water channel protein for red blood cells and kidney proximal tubule, Aquaporin-1, Chip28

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin.

Gene Name: Aqp1

Gen-ID: 25240

NCBI Accession: NP_036910

UniProt: P29975

Pathways: Hormone Transport