Bassoon Antikörper (N-Term)

Produktnummer ABIN6657297

Produktdetails anti-Bassoon Antikörper

Target: Bassoon (BSN)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Bassoon Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Verwendungszweck: BASSOON Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Bassoon from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Bassoon Antibody was purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: Bassoon Antibody was produced from whole rabbit serum prepared by repeated immunizations with a N-terminal his-tagged fusion protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 1:400

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Bassoon Antibody is tested for use in WB and IHC. Expect a band approximately ~420kDa on specific lysates corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. Specific conditions for reactivity should be optimized by the end user. Product provided in PBS pH 7.4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Bassoon

Target: Bassoon (BSN)

Andere Bezeichnung: Bassoon (BSN Produkte)

Synonyme: Znf231 antikoerper, ZNF231 antikoerper, bassoon antikoerper, bassoon (presynaptic cytomatrix protein) antikoerper, bassoon presynaptic cytomatrix protein antikoerper, Bsn antikoerper, BSN antikoerper

Hintergrund:

Synonyms: BSN, ZNF231, Neuronal double zine finger protein, protein bassoon

Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.

Gene Name: BSN

Gen-ID: 8927

NCBI Accession: NP_003449

UniProt: Q9UPA5