ATM Antikörper (pSer1981)

Produktnummer ABIN6657494

Hersteller Produkt- Nr.: 200-301-400s

Dieses Anti-ATM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ATM in WB, IHC, ELISA, IF, IP, FACS und FM. Geeignet für Human. Dieses Primary Antibody wurde in 124+ Publikationen zitiert.

Kurzübersicht für ATM Antikörper (pSer1981) (ABIN6657494)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Klon: 10H11-E12

Produktdetails anti-ATM Antikörper

Bindungsspezifität: AA 1974-1988, pSer1981

Hersteller Produkt- Nr.: 200-301-400s

Hersteller: Rockland

Verwendungszweck: ATM phospho S1981 Antibody

Kreuzreaktivität (Details): This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.

Aufreinigung: Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays.

Sterilität: Sterile filtered

Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:20,000 - 1:100,000

Immunohistochemistry_Dilution: Not Recommended

Flow_Cytometry_Dilution: 5 μg/mL

IF_Microscopy_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:200 - 1:2,000

Other: User Optimized

Kommentare:

Suggested Applications:

Suggested_Applications: Biochemical Assay, ChIP, FISH, IHC, IP, Multiplex

Other_Performance_Data: iFISH at 7.5μg/ml

Protein A Purified Mab anti-ATM has been tested by ELISA, FC, IF, and Western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells. By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (item# 200-301-500).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-ATM Antikörper (ABIN6657494)

Pan, Wu, Yeh: "ATM Inhibitor Suppresses Gemcitabine-Resistant BTC Growth in a Polymerase θ Deficiency-Dependent Manner." in: Biomolecules, Vol. 10, Issue 11, (2024) (PubMed).

Li, Gai, Li, Yang, Yu: "DNA-PK participates in pre-rRNA biogenesis independent of DNA double-strand break repair." in: Nucleic acids research, Vol. 52, Issue 11, pp. 6360-6375, (2024) (PubMed).

Xin, Gai, Ma, Li, Li, Yu: "Pre-rRNA Facilitates TopBP1-Mediated DNA Double-Strand Break Response." in: Advanced science (Weinheim, Baden-Wurttemberg, Germany), Vol. 10, Issue 28, pp. e2206931, (2023) (PubMed).

Chen, Li, Iemura, Tanaka: "Oxidative stress induces chromosomal instability through replication stress in fibroblasts from aged mice." in: Journal of cell science, Vol. 136, Issue 11, (2023) (PubMed).

Gritti, Basso, Rinchai, Corigliano, Pivetti, Gaviraghi, Rosano, Mazza, Barozzi, Roncador, Parmigiani, Legube, Parazzoli, Cittaro, Bedognetti, Mondino, Segalla, Tonon: "Loss of ribonuclease DIS3 hampers genome integrity in myeloma by disrupting DNA:RNA hybrid metabolism." in: The EMBO journal, Vol. 41, Issue 22, pp. e108040, (2022) (PubMed).

Wang, Oda, Suzuki, Mitani, Aoki: "Cell cycle-dependent radiosensitivity in mouse zygotes." in: DNA repair, Vol. 117, pp. 103370, (2022) (PubMed).

Montales, Ruis, Lindsay, Michael: "MRN-dependent and independent pathways for recruitment of TOPBP1 to DNA double-strand breaks." in: PloS one, Vol. 17, Issue 8, pp. e0271905, (2022) (PubMed).

Ka, Lim, Hwang, Kim, Lee: "IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer." in: Cell reports, Vol. 37, Issue 12, pp. 110138, (2022) (PubMed).

Wang, Lee, Shen, Lin, Wu, Lin, Cheng, Chang, Hung, Cho, Liu, Xia, Ji, Liang, Chiang, Liu, Yao, Hung, Wang: "Evading immune surveillance via tyrosine phosphorylation of nuclear PCNA." in: Cell reports, Vol. 36, Issue 8, pp. 109537, (2022) (PubMed).

Qi, Chakravarthy, Li, Deng, Li, Hu: "Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop." in: iScience, Vol. 25, Issue 9, pp. 104944, (2022) (PubMed).

Tanaka, Kondo, Fujita, Homma, Tagawa, Jin, Jin, Yoshioka, Takayama, Masuda, Tokuyama, Nakazaki, Saito, Saido, Murayama, Ikura, Ito, Yamamori, Tomii, Bianchi, Okazawa: "HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology." in: Communications biology, Vol. 4, Issue 1, pp. 1175, (2021) (PubMed).

Nguyen, Dzulko, Murr, Yen, Schneider, Krämer: "Class 1 Histone Deacetylases and Ataxia-Telangiectasia Mutated Kinase Control the Survival of Murine Pancreatic Cancer Cells upon dNTP Depletion." in: Cells, Vol. 10, Issue 10, (2021) (PubMed).

Homma, Tanaka, Jin, Jin, Huang, Yoshioka, Bertens, Tsumaki, Kondo, Shiwaku, Tagawa, Akatsu, Atsuta, Katsuno, Furukawa, Ishiki, Waragai, Ohtomo, Iwata, Yokota, Inoue, Arai, Sobue, Sone, Fujita, Okazawa: "DNA damage in embryonic neural stem cell determines FTLDs' fate via early-stage neuronal necrosis." in: Life science alliance, Vol. 4, Issue 7, (2021) (PubMed).

Qi, Ma, Naeem, Gu, Chao, Yuan, Huang: "Pb induced mitochondrial fission of fibroblast cells via ATM activation." in: Journal of hazardous materials, Vol. 416, pp. 126177, (2021) (PubMed).

Montales, Kim, Ruis, Michael: "Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) system." in: Scientific reports, Vol. 11, Issue 1, pp. 467, (2021) (PubMed).

Cabrini, Roncador, Galbiati, Cipolla, Maffia, Iannelli, Sabbioneda, d'Adda di Fagagna, Francia: "DROSHA is recruited to DNA damage sites by the MRN complex to promote non-homologous end joining." in: Journal of cell science, Vol. 134, Issue 6, (2021) (PubMed).

Tao, Aparicio, Li, Leong, Zha, Gautier: "Inhibition of DNA replication initiation by silver nanoclusters." in: Nucleic acids research, Vol. 49, Issue 9, pp. 5074-5083, (2021) (PubMed).

Zhu, Rogers, Asleh, Won, Gao, Leung, Li, Vij, Zhu, Held, You, Nielsen, Shao: "Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer." in: Cell reports, Vol. 31, Issue 10, pp. 107745, (2021) (PubMed).

Ishimoto, Tsuzuki, Matsumura, Kurashige, Enokitani, Narimatsu, Higa, Sugimoto, Yoshida, Fujita: "SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions." in: The Journal of cell biology, Vol. 220, Issue 1, (2021) (PubMed).

Deville, Luft, Kaufmann, Cordes: "Keap1 inhibition sensitizes head and neck squamous cell carcinoma cells to ionizing radiation via impaired non-homologous end joining and induced autophagy." in: Cell death & disease, Vol. 11, Issue 10, pp. 887, (2021) (PubMed).

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM

Hintergrund:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Gen-ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus