ATM Antikörper (pSer1981)

Produktnummer ABIN6657494

Produktdetails anti-ATM Antikörper

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Bindungsspezifität: AA 1974-1988, pSer1981

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Cross reactivity with ATM from other mammalian sources has not been tested.

Aufreinigung: Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000, whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human and mouse ATM.

Immunogen:

Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Klon: 10H11-E12

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: Not Recommended

Application Note: Protein A Purified Mab anti-ATM has been tested by ELISA and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and mouse cells. By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:200 - 1:2,000

IF Microscopy Dilution: 1:100 - 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Referenzen für anti-ATM Antikörper (ABIN6657494)

Kreis, Gallrein, Rojas-Puente, Mack, Kroon, Dinkel, Willmes, Murk, Tom-Dieck, Schuman, Kirstein, Eickholt: "ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans." in: Nature communications, Vol. 10, Issue 1, pp. 486, (2019) (PubMed).

Carvalho, Vítor, Sridhara, Martins, Raposo, Desterro, Ferreira, de Almeida: "SETD2 is required for DNA double-strand break repair and activation of the p53-mediated checkpoint." in: eLife, Vol. 3, pp. e02482, (2015) (PubMed).

Bartkova, Horejsí, Koed, Krämer, Tort, Zieger, Guldberg, Sehested, Nesland, Lukas, Ørntoft, Lukas, Bartek: "DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis." in: Nature, Vol. 434, Issue 7035, pp. 864-70, (2005) (PubMed).

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM (ATM Produkte)

Synonyme: ATM antikoerper, Atm antikoerper, CG6535 antikoerper, Dmel\\CG6535 antikoerper, Tefu antikoerper, atm antikoerper, atm/tefu antikoerper, dATM antikoerper, tef antikoerper, Xatm antikoerper, at1 antikoerper, atdc antikoerper, tel1 antikoerper, telo1 antikoerper, AT1 antikoerper, ATA antikoerper, ATC antikoerper, ATD antikoerper, ATDC antikoerper, ATE antikoerper, TEL1 antikoerper, TELO1 antikoerper, AI256621 antikoerper, C030026E19Rik antikoerper, telomere fusion antikoerper, ATM serine/threonine kinase L homeolog antikoerper, ATM serine/threonine kinase antikoerper, ataxia telangiectasia mutated antikoerper, ataxia telangiectasia mutated (atm) antikoerper, serine/threonine-protein kinase ATM antikoerper, tefu antikoerper, atm.L antikoerper, atm antikoerper, ATM antikoerper, EDI_100660 antikoerper, CpipJ_CPIJ001772 antikoerper, BDBG_08252 antikoerper, PAAG_02532 antikoerper, MCYG_05088 antikoerper, VDBG_06833 antikoerper, ACLA_015700 antikoerper, LOC5565620 antikoerper, MGYG_07634 antikoerper, PGTG_14279 antikoerper, Atm antikoerper

Hintergrund:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus