ZIC1 Antikörper (N-Term)

Produktnummer ABIN6657599

Hersteller Produkt- Nr.: 200-401-159

Produktdetails anti-ZIC1 Antikörper

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Bindungsspezifität: N-Term

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZIC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-401-159

Hersteller: Rockland

Verwendungszweck: Zic-1 Antibody

Kreuzreaktivität (Details): This antibody is directed against Zic-1 from mouse.

Aufreinigung: This is an IgG preparation of whole rabbit antiserum purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with an 18 aa synthetic peptide from a region near the N-Terminus of mouse Zic-1. This domain is completely conserved in human ZIC-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:400

IF_Microscopy_Dilution: 1:400

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Kommentare:

Suggested Applications: IF, Multiplex
Anti Zic-1 Antibody has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ZIC1 Antikörper (ABIN6657599)

He, Yu, Lu, Wang, Wu, Zhao, Li, Zhou, Liu, Mu, Xin, Qiu, Lu: "Transcriptional Regulator ZEB2 Is Essential for Bergmann Glia Development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 6, pp. 1575-1587, (2019) (PubMed).

Sankar, Yellajoshyula, Zhang, Teets, Rockweiler, Kroll: "Gene regulatory networks in neural cell fate acquisition from genome-wide chromatin association of Geminin and Zic1." in: Scientific reports, Vol. 6, pp. 37412, (2018) (PubMed).

He, Zhang, Chen, Remke, Shih, Lu, Wang, Deng, Yu, Xia, Wu, Ramaswamy, Hu, Wang, Zhou, Burns, Kim, Kool, Pfister, Weinstein, Pomeroy, Gilbertson, Rubin, Hou, Wechsler-Reya, Taylor, Lu: "The G protein α subunit Gαs is a tumor suppressor in Sonic hedgehog-driven medulloblastoma." in: Nature medicine, Vol. 20, Issue 9, pp. 1035-42, (2014) (PubMed).

Zhao, Ayrault, Zindy, Kim, Roussel: "Post-transcriptional down-regulation of Atoh1/Math1 by bone morphogenic proteins suppresses medulloblastoma development." in: Genes & development, Vol. 22, Issue 6, pp. 722-7, (2008) (PubMed).

Details zu ZIC1

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Andere Bezeichnung: Zic-1 (ZIC1 Produkte)

Hintergrund:

Synonyms: rabbit anti-Zic-1 Antibody, Odd paired homolog Drosophila antibody, Zic 1 antibody, ZIC antibody, Zic family member 1 (odd-paired Drosophila homolog) antibody, Zic family member 1 antibody

Background: Anti Zic-1 Antibody recognizes the product of the Zic-1 gene, that encodes a zinc finger protein which is expressed in the developing or matured central nervous system in a highly restricted manner.  Zic-1 is expressed in granule cells that make synaptic contact with Purkinje cells.  Clearly Zic-1 is a gene critical to cerebellar pattern formation.  The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm.  Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family.  By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation.  The Zic-1 gene has been mapped to chromosome 9 in mouse.  The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis.  The temporal profile of mRNA expression differs for each of the Zic gene products.  The Drosophila odd-paired gene is highly homologous to the Zic gene family.

Gene Name: Zic1

Gen-ID: 22771

UniProt: P46684