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MCL-1 Antikörper (Internal Region)

Rockland 600-401-394s MCL1 Reaktivität: Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN6657787
Hersteller Produkt- Nr.: 600-401-394s
  • Target Alle MCL-1 (MCL1) Antikörper anzeigen
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Bindungsspezifität
    • 27
    • 16
    • 16
    • 11
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 141
    • 64
    • 62
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Maus
    Wirt
    • 132
    • 12
    • 1
    • 1
    Kaninchen
    Klonalität
    • 125
    • 23
    Polyklonal
    Konjugat
    • 71
    • 9
    • 9
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser MCL-1 Antikörper ist unkonjugiert
    Applikation
    • 115
    • 55
    • 46
    • 43
    • 27
    • 26
    • 23
    • 22
    • 11
    • 9
    • 7
    • 5
    • 4
    Western Blotting (WB), ELISA
    Hersteller Produkt- Nr.
    600-401-394s
    Hersteller
    Rockland
    Verwendungszweck
    Mcl-1 Antibody
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
    Aufreinigung
    Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.
    Sterilität
    Sterile filtered
    Immunogen
    This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
    Isotyp
    IgG
    Top Product
    Discover our top product MCL1 Primärantikörper
  • Applikationshinweise

    ELISA_Dilution: 1:10,000 - 1:50,000

    Western_Blot_Dilution: 1:10,000

    Other: User Optimized

    Kommentare

    Suggested Applications: FC, IF, IHC, IP
    Anti-Mcl-1 Antibody has been tested by ELISA and western blot and is suitable for immunoprecipitation.  This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence.  Cross reactivity with Mcl-1 from other sources is unknown.  This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size.  The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Karbon, Schuler, Braun, Eichin, Haschka, Drach, Sotillo, Geley, Spierings, Tijhuis, Foijer, Villunger: "Chronic spindle assembly checkpoint activation causes myelosuppression and gastrointestinal atrophy." in: EMBO reports, Vol. 25, Issue 6, pp. 2743-2772, (2024) (PubMed).

    Lindenboim, Zohar, Gundersen, Worman, Stein: "LINC complex protein nesprin-2 has pro-apoptotic activity via Bcl-2 family proteins." in: Cell death discovery, Vol. 10, Issue 1, pp. 29, (2024) (PubMed).

    Prucsi, Zimny, Płonczyńska, Zubrzycka, Potempa, Sochalska: "Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis." in: International journal of molecular sciences, Vol. 24, Issue 16, (2023) (PubMed).

    Quarato, Mari, Barrows, Yang, Ruehl, Chen, Guy, Low, Chen, Green: "Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis." in: Science advances, Vol. 9, Issue 21, pp. eadg8156, (2023) (PubMed).

    Huang, Chin, Reljic, Djajawi, Tan, Gong, Stroud, Huang, van Delft, Dewson: "Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins." in: Cell death and differentiation, Vol. 30, Issue 3, pp. 632-646, (2023) (PubMed).

    Pileggi, Parmar, Elkhatib, Stewart, Alecu, Côté, Bennett, Sandhu, Cuperlovic-Culf, Harper: "The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics." in: Current research in neurobiology, Vol. 5, pp. 100112, (2023) (PubMed).

    Prew, Adhikary, Choi, Portero, Paulo, Gowda, Budhraja, Opferman, Gygi, Danial, Walensky: "MCL-1 is a master regulator of cancer dependency on fatty acid oxidation." in: Cell reports, Vol. 41, Issue 1, pp. 111445, (2022) (PubMed).

    Quarato, Llambi, Guy, Min, Actis, Sun, Narina, Pruett-Miller, Peng, Rankovic, Green: "Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak." in: Cell death and differentiation, Vol. 29, Issue 7, pp. 1318-1334, (2022) (PubMed).

    Moyzis, Lally, Liang, Najor, Gustafsson: "Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage." in: Cells, Vol. 11, Issue 9, (2022) (PubMed).

    Buitrago-Molina, Marhenke, Becker, Geffers, Itzel, Teufel, Jaeschke, Lechel, Unger, Markovic, Sharma, Marquardt, Saborowski, Saborowski, Vogel: "p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model." in: Cellular and molecular gastroenterology and hepatology, Vol. 11, Issue 5, pp. 1387-1404, (2022) (PubMed).

    Smith, Budhraja, Lynch, Roberts, Panetta, Connelly, Turnis, Pruett-Miller, Schuetz, Mullighan, Opferman: "The Heme-Regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics." in: Molecular cancer research : MCR, Vol. 19, Issue 4, pp. 636-650, (2022) (PubMed).

    Teh, Robbins, Henstridge, Dewson, Diepstraten, Kelly, Febbraio, Gabriel, O'Reilly, Strasser, Gray: "MCL-1 is essential for survival but dispensable for metabolic fitness of FOXP3+ regulatory T cells." in: Cell death and differentiation, Vol. 27, Issue 12, pp. 3374-3385, (2021) (PubMed).

    Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

    Djajawi, Liu, Gong, Huang, Luo, Xu, Okamoto, Call, Huang, van Delft: "MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex." in: Cell death and differentiation, Vol. 27, Issue 8, pp. 2484-2499, (2021) (PubMed).

    Moyzis, Lally, Liang, Leon, Najor, Orogo, Gustafsson: "Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise." in: Journal of molecular and cellular cardiology, Vol. 146, pp. 109-120, (2021) (PubMed).

    Mizutani, Hikita, Saito, Myojin, Sato, Urabe, Kurahashi, Shiode, Sakane, Murai, Nozaki, Kodama, Sakamori, Yoshida, Tatsumi, Takehara: "Gab1 in livers with persistent hepatocyte apoptosis has an antiapoptotic effect and reduces chronic liver injury, fibrosis, and tumorigenesis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 320, Issue 6, pp. G958-G968, (2021) (PubMed).

    Sumiyoshi, Kotani, Ikuta, Suzue, Ozawa, Katakai, Yamada, Abe, Bando, Koyasu, Kanaho, Watanabe, Matsuda: "Arf1 and Arf6 Synergistically Maintain Survival of T Cells during Activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 206, Issue 2, pp. 366-375, (2021) (PubMed).

    Hirsova, Bohm, Dohnalkova, Nozickova, Heikenwalder, Gores, Weber: "Hepatocyte apoptosis is tumor promoting in murine nonalcoholic steatohepatitis." in: Cell death & disease, Vol. 11, Issue 2, pp. 80, (2021) (PubMed).

    Jayavelu, Schnöder, Perner, Herzog, Meiler, Krishnamoorthy, Huber, Mohr, Edelmann-Stephan, Austin, Brandt, Palandri, Schröder, Isermann, Edlich, Sinha, Ungelenk, Hübner, Zeiser, Rahmig, Waskow, Coldham, Ernst, Hochhaus, Jilg, Jost, Mullally, Bullinger, Me: "Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms." in: Nature, Vol. 588, Issue 7836, pp. 157-163, (2021) (PubMed).

    Patel, Mendoza, Robichaux, Wang, Wehrens, Karch: "Inhibition of the Anti-Apoptotic Bcl-2 Family by BH3 Mimetics Sensitize the Mitochondrial Permeability Transition Pore Through Bax and Bak." in: Frontiers in cell and developmental biology, Vol. 9, pp. 765973, (2021) (PubMed).

  • Target
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Andere Bezeichnung
    Mcl-1 (MCL1 Produkte)
    Hintergrund

    Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

    Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

    Gene Name: Mcl1

    Gen-ID
    17210
    UniProt
    P97287
    Pathways
    MAPK Signalweg
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