CD3 epsilon Antikörper (FITC)

Produktnummer ABIN6657857

Hersteller Produkt- Nr.: 200-b02-m69

Produktdetails anti-CD3 epsilon Antikörper (FITC)

Target: CD3 epsilon (CD3E)

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Hersteller Produkt- Nr.: 200-b02-m69

Hersteller: Rockland

Verwendungszweck: CD3e Fluorescein Antibody

Kreuzreaktivität (Details): Reactivity is observed against mouse CD3.

Aufreinigung: Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3.

Sterilität: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.

Klon: 145-2C11

Isotyp: IgG

Labeling Ratio: 2-8

Anwendungsinformationen

Applikationshinweise:

Flow_Cytometry_Dilution: 0.1-0.5 μg/10^6 cells

Other: Sufficient to run approximately 500 tests

Kommentare:

Anti-CD3 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e (CD3E Produkte)

Hintergrund:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.

Gene Name: Cd3e

Gen-ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway