TCRb Antikörper (FITC)

Produktnummer ABIN6657906

Hersteller Produkt- Nr.: 200-b02-n92

Produktdetails anti-TCRb Antikörper (FITC)

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser TCRb Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-b02-n92

Hersteller: Rockland

Verwendungszweck: TCRbeta Fluorescein Antibody

Kreuzreaktivität (Details): Cross reactivity with TCRbeta from other sources has not been tested.

Aufreinigung: Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta.

Sterilität: Sterile filtered

Immunogen: Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.

Klon: H57-597

Isotyp: IgG

Labeling Ratio: 2-8

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Other: Sufficient to run approximately 500 tests

Kommentare:

Anti-TCR beta is tested for FLOW and useful for Immunofluorescence and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu TCRb

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Andere Bezeichnung: Tcrb (TCRb Produkte)

Hintergrund:

Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

Background: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

Gene Name: Tcrb

Gen-ID: 21577