SIX3 Antikörper (Internal Region)

Produktnummer ABIN6657910

Hersteller Produkt- Nr.: 200-201-a26

Produktdetails anti-SIX3 Antikörper

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Bindungsspezifität: Internal Region

Reaktivität: Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser SIX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-201-a26

Hersteller: Rockland

Verwendungszweck: SIX3 Antibody

Kreuzreaktivität (Details): This antibody reacts with mouse Six3.

Aufreinigung: This product was purified by Protein A chromatography from monospecific antiserum.

Sterilität: Sterile filtered

Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:5,000 - 1:25,000

Immunohistochemistry_Dilution: 1:250 - 1:500

IF_Microscopy_Dilution: 1:250 - 1:500

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Kommentare:

Suggested Applications: Multiplex
This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SIX3 Antikörper (ABIN6657910)

Madrigal, Moreno-Bravo, Martínez-López, Martínez, Puelles: "Mesencephalic origin of the rostral Substantia nigra pars reticulata." in: Brain structure & function, Vol. 221, Issue 3, pp. 1403-12, (2016) (PubMed).

Moldrich, Gobius, Pollak, Zhang, Ren, Brown, Mori, De Juan Romero, Britanova, Tarabykin, Richards: "Molecular regulation of the developing commissural plate." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3645-61, (2010) (PubMed).

Details zu SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Andere Bezeichnung: SIX3 (SIX3 Produkte)

Hintergrund:

Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: SIX3

Gen-ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus