SIX3 Antikörper (Internal Region)

Produktnummer ABIN6657911

Hersteller Produkt- Nr.: 200-201-a26s

Dieses Anti-SIX3-Antikörper ist ein Meerschweinchen Polyklonal-Antikörper zur Detektion von SIX3 in WB, ELISA, IHC, IF, FM und DB. Geeignet für Maus. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für SIX3 Antikörper (Internal Region) (ABIN6657911)

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reaktivität: Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser SIX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Dot Blot (DB)

Produktdetails anti-SIX3 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 200-201-a26s

Hersteller: Rockland

Verwendungszweck: SIX3 Antibody

Kreuzreaktivität (Details): This antibody reacts with mouse Six3.

Aufreinigung: This product was purified by Protein A chromatography from monospecific antiserum.

Sterilität: Sterile filtered

Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:5,000 - 1:25,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Kommentare:

Suggested Applications: Multiplex
This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 3 months

Referenzen für anti-SIX3 Antikörper (ABIN6657911)

Lipiec, Bem, Koziński, Chakraborty, Urban-Ciećko, Zajkowski, Dąbrowski, Szewczyk, Toval, Ferran, Nagalski, Wiśniewska: "TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." in: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).

Grall, Gourain, Naïr, Martin, Birling, Freund, Duluc: "Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs." in: Cell death & disease, Vol. 10, Issue 11, pp. 812, (2020) (PubMed).

OBrien, Guo, Bahrami-Samani, Park, Hasso, Lee, Fang, Kim, Guo, Hong, Peterson, Lozanoff, Raviram, Ren, Fogelgren, Smith, Valouev, McMahon: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." in: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).

Madrigal, Moreno-Bravo, Martínez-López, Martínez, Puelles: "Mesencephalic origin of the rostral Substantia nigra pars reticulata." in: Brain structure & function, Vol. 221, Issue 3, pp. 1403-12, (2016) (PubMed).

Moldrich, Gobius, Pollak, Zhang, Ren, Brown, Mori, De Juan Romero, Britanova, Tarabykin, Richards: "Molecular regulation of the developing commissural plate." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3645-61, (2010) (PubMed).

Details zu SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Andere Bezeichnung: SIX3

Hintergrund:

Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: SIX3

Gen-ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus