SHANK1 Antikörper (Internal Region)

Produktnummer ABIN6658104

Produktdetails anti-SHANK1 Antikörper

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Bindungsspezifität: Internal Region

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SHANK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Anti-SHANK1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SHANK1 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against Shank2 or Shank3. Cross-reactivity with Shank1 from other sources has not been determined. Scaffolds research.

Immunogen:

Immunogen: SHANK1 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an internal region of (SH3/PDZ domains) of rat Shank1.

Immunogen Type: Recombinant Protein

Klon: S22-21

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-SHANK1 Antibody is suitable for use in WB and IHC. Expect a band approximately ~190-220 kDa (alternative splice variants) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu SHANK1

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Andere Bezeichnung: SHANK1 (SHANK1 Produkte)

Synonyme: SPANK-1 antikoerper, SSTRIP antikoerper, synamon antikoerper, Shank1a antikoerper, Spank1 antikoerper, Sstrip antikoerper, SH3 and multiple ankyrin repeat domains 1 antikoerper, SHANK1 antikoerper, Shank1 antikoerper

Hintergrund:

Synonyms: Spank1, Sstrip, Shank1a, Shank1, SH3 and multiple ankyrin repeat domains protein 1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank1 specifically might be relevant to human autism spectrum disorders, due to its differential role in specific cognitive processes, along with its importance for synapse structure and function in vivo.

Gene Name: Spank1

Gen-ID: 78957

NCBI Accession: NP_113939

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location