BrdU Antikörper

Produktnummer ABIN669246

Produktdetails anti-BrdU Antikörper

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Kreuzreaktivität: Human, Ratte

Kreuzreaktivität (Details): BrdU

Aufreinigung: Purified by Protein A.

Immunogen: BrdU conjugated to KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-BrdU Antikörper (ABIN669246)

Giuliani, Neri, Canalini, Calevro, Ottani, Vandini, Sena, Zaffe, Guarini: "NDP-?-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC4 receptors." in: Molecular and cellular neurosciences, Vol. 67, pp. 13-21, (2015) (PubMed).

Chen, Li, Xu, Yin: "Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 34, Issue 4, pp. 1109-24, (2015) (PubMed).

Zhang, Zhang, Fang, Li, Zhao, Shi, An: "Neuroprotective effects of sulforaphane on cholinergic neurons in mice with Alzheimer's disease-like lesions." in: International journal of molecular sciences, Vol. 15, Issue 8, pp. 14396-410, (2014) (PubMed).

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU (BrdU Produkte)

Substanzklasse: Chemical

Hintergrund:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis