BCL9L Antikörper (AA 397-666)

Produktnummer ABIN6719451

Produktdetails anti-BCL9L Antikörper

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Bindungsspezifität: AA 397-666

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCL9L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BCL9L recombinant protein (Position: E397-A666).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu BCL9L

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Andere Bezeichnung: BCL9L (BCL9L Produkte)

Synonyme: BCL9-2 antikoerper, DLNB11 antikoerper, B9L antikoerper, BC003321 antikoerper, bcl9-2 antikoerper, BCL9L antikoerper, B-cell CLL/lymphoma 9 like antikoerper, B cell CLL/lymphoma 9-like antikoerper, B-cell CLL/lymphoma 9-like antikoerper, BCL9L antikoerper, Bcl9l antikoerper, bcl9l antikoerper

Hintergrund:

Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11

Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9, also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391-1426. Over aa 1009-1328, human Bcl-9 is 96 % aa identical to mouse Bcl-9.

Gen-ID: 283149

Pathways: Stem Cell Maintenance