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TRAC Antikörper (AA 1-114)

TRAC Reaktivität: Human, Maus, Ratte ELISA, WB, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6719654
  • Target Alle TRAC Produkte
    TRAC (T Cell Receptor alpha Constant (TRAC))
    Bindungsspezifität
    • 5
    • 4
    AA 1-114
    Reaktivität
    Human, Maus, Ratte
    Wirt
    • 9
    Kaninchen
    Klonalität
    • 9
    Polyklonal
    Konjugat
    • 3
    • 2
    • 2
    • 2
    Dieser TRAC Antikörper ist unkonjugiert
    Applikation
    ELISA, Western Blotting (WB), Flow Cytometry (FACS)
    Verwendungszweck
    Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.
    Kreuzreaktivität (Details)
    No cross reactivity with other proteins.
    Produktmerkmale
    Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).
    Isotyp
    IgG
  • Applikationshinweise

    Application details: Western blot|0.1-0.5 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

    Kommentare

    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    TRAC (T Cell Receptor alpha Constant (TRAC))
    Andere Bezeichnung
    TRAC (TRAC Produkte)
    Synonyme
    TCRA antikoerper, Gm16914 antikoerper, Tcra antikoerper, Tcra-C antikoerper, T-cell receptor alpha constant antikoerper, T cell receptor alpha constant antikoerper, TRAC antikoerper, Trac antikoerper
    Hintergrund

    Synonyms: T-cell receptor alpha chain C region, TRAC, TCRA

    Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

    Gen-ID
    28755
    UniProt
    P01848
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