His Tag Antikörper (DyLight 405)

Produktnummer ABIN6720710

Hersteller Produkt- Nr.: 200-346-382

Produktdetails anti-His Tag Antikörper (DyLight 405)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit DyLight 405

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Hersteller Produkt- Nr.: 200-346-382

Hersteller: Rockland

Verwendungszweck: 6X His Tag Antibody Dylight™ 405 Conjugated

Kreuzreaktivität (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Aufreinigung: 6X His Tag Antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Klon: 33D10-D2-G8

Isotyp: IgG1 kappa

Labeling Ratio: 2.4

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Kommentare:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store 6X His Tag Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Abstract: His Tag Produkte

Hintergrund:

Synonyms: mouse anti-6X His Tag biotin conjugated Antibody, biotin conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.