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Rekombinanter Rpb1 CTD Antikörper (pTyr1)

Reaktivität: Human WB, IF, ELISA Wirt: Maus Monoclonal unconjugated Recombinant Antibody
Produktnummer ABIN6731014
  • Target Alle Rpb1 CTD Produkte
    Rpb1 CTD (RNA Polymerase II CTD)
    Antikörpertyp
    Recombinant Antibody
    Bindungsspezifität
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    pTyr1
    Reaktivität
    • 24
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 11
    • 9
    • 4
    Maus
    Klonalität
    • 15
    • 9
    Monoklonal
    Konjugat
    • 24
    Dieser Rpb1 CTD Antikörper ist unkonjugiert
    Applikation
    • 24
    • 17
    • 15
    • 10
    • 7
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA
    Marke
    AbFlex®
    Aufreinigung
    Protein A Chromatography
    Immunogen
    This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
    Isotyp
    IgG2a
  • Applikationshinweise

    Validated Applications:
    IF: 0.5 - 2 µg/ml
    Bead-based ELISA: 0.5 - 10 µg/ml
    WB: 1 - 2 μg/mL

    For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.
    Kommentare

    AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
  • Target
    Rpb1 CTD (RNA Polymerase II CTD)
    Andere Bezeichnung
    RNA Pol II CTD (Rpb1 CTD Produkte)
    Molekulargewicht
    210 kDa
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