Rekombinanter Rpb1 CTD Antikörper (pTyr1)
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- Target Alle Rpb1 CTD Produkte
- Rpb1 CTD (RNA Polymerase II CTD)
- Antikörpertyp
- Recombinant Antibody
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Bindungsspezifität
- pTyr1
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Rpb1 CTD Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- Marke
- AbFlex®
- Aufreinigung
- Protein A Chromatography
- Immunogen
- This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
- Isotyp
- IgG2a
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- Applikationshinweise
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Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL
For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required. - Kommentare
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
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- Target
- Rpb1 CTD (RNA Polymerase II CTD)
- Andere Bezeichnung
- RNA Pol II CTD (Rpb1 CTD Produkte)
- Molekulargewicht
- 210 kDa
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