CHEK1 Antikörper (AA 192-241)

Produktnummer ABIN6736365

Produktdetails anti-CHEK1 Antikörper

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Bindungsspezifität: AA 192-241

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Affe, Zebrafisch (Danio rerio), Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CHEK1 / CHK1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse (100%) Bovine, Pig, Guinea pig, Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa192-241 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Zebra finch (100%), Hamster, Bovine, Pig, Opossum, Guinea pig, Chicken, Platypus (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHEK1 / CHK1 (CHEK1 Produkte)

Hintergrund: Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

Gen-ID: 1111

NCBI Accession: NP_001265

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur