HNF4A Antikörper (N-Term)

Produktnummer ABIN214775

Produktdetails anti-HNF4A Antikörper

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Affe, Pferd, Kaninchen, Meerschweinchen, Schaf, Fledermaus, Hamster, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNF4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human HNF4A / HNF4, immunogenic peptide sequence is present in isoforms 1, 2, 3, and 4.

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HNF4A (P41235, NP_000448). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%), Catfish, Stickleback, Zebrafish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Andere Bezeichnung: HNF4A / HNF4 (HNF4A Produkte)

Synonyme: HNF4A antikoerper, HNF4alpha antikoerper, CG9310 antikoerper, DmHNF4 antikoerper, Dmel\\CG9310 antikoerper, HNF-4 antikoerper, HNF-4(D) antikoerper, HNF4 antikoerper, Hnf-4 antikoerper, Hnf-4h antikoerper, NR2A4 antikoerper, dHNF-4 antikoerper, dHNF4 antikoerper, Hnf4 antikoerper, Hnf4alpha antikoerper, MODY1 antikoerper, Nr2a1 antikoerper, Tcf14 antikoerper, HNF4a7 antikoerper, HNF4a8 antikoerper, HNF4a9 antikoerper, MODY antikoerper, NR2A1 antikoerper, NR2A21 antikoerper, TCF antikoerper, TCF14 antikoerper, HNF-4alpha antikoerper, fb58h09 antikoerper, id:ibd1279 antikoerper, wu:fb58h09 antikoerper, hnf4 antikoerper, nr2a1 antikoerper, hepatocyte nuclear factor 4 alpha antikoerper, Hepatocyte nuclear factor 4 antikoerper, hepatic nuclear factor 4, alpha antikoerper, hepatocyte nuclear factor 4, alpha antikoerper, hepatocyte nuclear factor 4 alpha S homeolog antikoerper, HNF4A antikoerper, hnf4a antikoerper, Hnf4 antikoerper, Hnf4a antikoerper, hnf4a.S antikoerper

Hintergrund: Name/Gene ID: HNF4A
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: HNF4A, Hepatic nuclear factor 4 alpha, HNF4, HNF4a8, NR2A21, TCF, TCF-14, TCF14, MODY1, Transcription factor 14, Transcription factor HNF-4, HNF-4-alpha, HNF4 alpha, HNF4a7, HNF4a9, HNF4alpha, HNF4alpha10/11/12, MODY, NR2A1

Gen-ID: 3172

NCBI Accession: NP_000448

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process