PHD1 Antikörper (AA 58-107)

Produktnummer ABIN6736715

Produktdetails anti-PHD1 Antikörper

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Bindungsspezifität: AA 58-107

Reaktivität: Human, Rind (Kuh), Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human EGLN2 / PHD1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Guinea pig (85%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide located between aa58-107 of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse, Rat, Dog, Pig (92%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Andere Bezeichnung: EGLN2 / PHD1 (EGLN2 Produkte)

Synonyme: egln2 antikoerper, phd2 antikoerper, PHD1 antikoerper, 0610011A13Rik antikoerper, C85656 antikoerper, Hif-p4h-1 antikoerper, Ier4 antikoerper, Phd1 antikoerper, SM-20 antikoerper, HIF-PH1 antikoerper, HPH-1 antikoerper, HPH-3 antikoerper, PHD-1 antikoerper, EIT6 antikoerper, HIFPH1 antikoerper, egl-9 family hypoxia-inducible factor 1 antikoerper, egl-9 family hypoxia inducible factor 2 antikoerper, Egl nine homolog 2 antikoerper, egl-9 family hypoxia-inducible factor 2 L homeolog antikoerper, egl-9 family hypoxia-inducible factor 2 antikoerper, egln1 antikoerper, EGLN2 antikoerper, egln2 antikoerper, egln2.L antikoerper, Egln2 antikoerper

Hintergrund: Name/Gene ID: EGLN2

Synonyms: EGLN2, EIT6, EGL nine (C.elegans) homolog 2, Egl nine homolog 2, HPH-1, HPH-3, Estrogen-induced tag 6, PHD1, HIF-PH1, HIF-prolyl hydroxylase 1, HIFPH1

Gen-ID: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis