GATA6 Antikörper (C-Term)

Produktnummer ABIN462170

Produktdetails anti-GATA6 Antikörper

Target: GATA6 (GATA Binding Protein 6 (GATA6))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GATA6

Homologie: Percent identity by BLAST analysis: Human, Horse (100%) Sheep, Rabbit, Pig (92%) Rat, Bovine (85%) Guinea pig (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GATA6 (Q92908, NP_005248). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Horse (100%), Sheep, Rabbit, Pig (92%), Rat, Panda, Bovine (85%), Guinea pig (84%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IF

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu GATA6

Target: GATA6 (GATA Binding Protein 6 (GATA6))

Andere Bezeichnung: GATA6 (GATA6 Produkte)

Synonyme: AA410133 antikoerper, GATA-6 antikoerper, XGATA-6 antikoerper, XGATA6 antikoerper, gata6a antikoerper, gata6b antikoerper, pnr antikoerper, tGata6 antikoerper, gata6 antikoerper, gata-6 antikoerper, GATA-5 antikoerper, cb603 antikoerper, GATA binding protein 6 antikoerper, GATA binding protein 6 S homeolog antikoerper, GATA binding protein 6 L homeolog antikoerper, GATA6 antikoerper, Gata6 antikoerper, gata6 antikoerper, gata6.S antikoerper, gata6.L antikoerper

Hintergrund: Name/Gene ID: GATA6

Synonyms: GATA6, GATA-binding factor 6, GATA-binding protein 6, GATA binding protein 6, Transcription factor GATA-6

Gen-ID: 2627

NCBI Accession: NP_005248

UniProt: Q92908