PAX7 Antikörper (AA 167-216)

Produktnummer ABIN462176

Produktdetails anti-PAX7 Antikörper

Target: PAX7 (Paired Box 7 (PAX7))

Bindungsspezifität: AA 167-216

Reaktivität: Human, Maus, Ratte, Huhn, Hund, Meerschweinchen, Rind (Kuh), Kaninchen, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PAX7

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken (100%) Xenopus, Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa167-216 of human PAX7 (P23759, NP_002575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%), Xenopus, Salmon, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.05 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Andere Bezeichnung: PAX7 (PAX7 Produkte)

Hintergrund: Name/Gene ID: PAX7

Synonyms: PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Gen-ID: 5081

NCBI Accession: NP_002575

UniProt: P23759