NCOA3 Antikörper (AA 576-625)

Produktnummer ABIN6741542

Produktdetails anti-NCOA3 Antikörper

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Bindungsspezifität: AA 576-625

Reaktivität: Human, Rind (Kuh), Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NCOA3

Homologie: Percent identity by BLAST analysis: Human (100%) Horse, Pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa576-625 of human NCOA3 (Q0VF45, NP_006525). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Pig (100%), Horse (92%), Marmoset, Bat (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Andere Bezeichnung: NCOA3 / SRC-3 / AIB1 (NCOA3 Produkte)

Synonyme: NCOA3 antikoerper, ACTR antikoerper, AIB-1 antikoerper, AIB1 antikoerper, CAGH16 antikoerper, CTG26 antikoerper, KAT13B antikoerper, RAC3 antikoerper, SRC-3 antikoerper, SRC3 antikoerper, TNRC14 antikoerper, TNRC16 antikoerper, TRAM-1 antikoerper, bHLHe42 antikoerper, pCIP antikoerper, 2010305B15Rik antikoerper, AW321064 antikoerper, Actr antikoerper, Aib1 antikoerper, Rac3 antikoerper, Src3 antikoerper, Tram-1 antikoerper, Tram1 antikoerper, p/Cip antikoerper, pCip antikoerper, actr antikoerper, aib-1 antikoerper, aib1 antikoerper, cagh16 antikoerper, ctg26 antikoerper, kat13b antikoerper, pcip antikoerper, rac3 antikoerper, src-3 antikoerper, src3 antikoerper, tnrc14 antikoerper, tnrc16 antikoerper, tram-1 antikoerper, xSRC-3 antikoerper, nuclear receptor coactivator 3 antikoerper, nuclear receptor coactivator 3 S homeolog antikoerper, NCOA3 antikoerper, Ncoa3 antikoerper, ncoa3.S antikoerper

Hintergrund: Name/Gene ID: NCOA3

Synonyms: NCOA3, ACTR, AIB1, BHLHe42, CAGH16, CBP-interacting protein, CTG26, AIB-1, P/CIP, TNRC14, TRAM-1, Nuclear receptor coactivator 3, PCIP, RAC-3, SRC3, TNRC16, KAT13B, NCoA-3, SRC-3

Gen-ID: 8202

NCBI Accession: NP_006525

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha