TCF21 Antikörper (AA 42-91)

Produktnummer ABIN462190

Produktdetails anti-TCF21 Antikörper

Target: TCF21 (Transcription Factor 21 (TCF21))

Bindungsspezifität: AA 42-91

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Fledermaus, Hamster, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TCF21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human TCF21

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa42-91 of human TCF21 (O43680, NP_003197). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Gibbon, Marmoset (92%), Opossum, Turkey, Zebra finch, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TCF21

Target: TCF21 (Transcription Factor 21 (TCF21))

Andere Bezeichnung: TCF21 / Epicardin (TCF21 Produkte)

Hintergrund: Name/Gene ID: TCF21
Family: Transcription factor

Synonyms: TCF21, Capsulin, BHLHa23, Epicardin, Pod-1, Transcription factor 21, TCF-21, POD1, Podocyte-expressed 1

Gen-ID: 6943

NCBI Accession: NP_003197

UniProt: O43680

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling