ASH2L Antikörper (AA 288-337)

Produktnummer ABIN6741556

Produktdetails anti-ASH2L Antikörper

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Bindungsspezifität: AA 288-337

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Affe, Fledermaus, Kaninchen, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASH2L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ASH2L

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%) Bovine, Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa288-337 of human ASH2L (Q9UBL3, NP_004665). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%), Bovine, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Andere Bezeichnung: ASH2L / ASH2 (ASH2L Produkte)

Synonyme: ASH2 antikoerper, ASH2L1 antikoerper, ASH2L2 antikoerper, Bre2 antikoerper, fb34c06 antikoerper, fe38b04 antikoerper, fi20h02 antikoerper, wu:fb34c06 antikoerper, wu:fe38b04 antikoerper, wu:fi20h02 antikoerper, zgc:171444 antikoerper, ASH2L antikoerper, ash2-like antikoerper, ASH2 like histone lysine methyltransferase complex subunit antikoerper, ash2 (absent, small, or homeotic)-like (Drosophila) antikoerper, ash2 (absent, small, or homeotic)-like L homeolog antikoerper, ASH2L antikoerper, Ash2l antikoerper, ash2l antikoerper, ash2l.L antikoerper

Hintergrund: Name/Gene ID: ASH2L

Synonyms: ASH2L, ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2

Gen-ID: 9070

NCBI Accession: NP_004665

UniProt: Q9UBL3