RHOXF2 Antikörper (AA 108-157)

Produktnummer ABIN6741566

Produktdetails anti-RHOXF2 Antikörper

Target: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Bindungsspezifität: AA 108-157

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RHOXF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human RHOXF2

Homologie: Percent identity by BLAST analysis: Human (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human RHOXF2 (Q9BQY4, NP_115887). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%), Monkey (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu RHOXF2

Target: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Andere Bezeichnung: RHOXF2 (RHOXF2 Produkte)

Synonyme: CT107 antikoerper, PEPP-2 antikoerper, PEPP2 antikoerper, THG1 antikoerper, Rhox homeobox family member 2 antikoerper, RHOXF2 antikoerper

Hintergrund: Name/Gene ID: RHOXF2

Synonyms: RHOXF2, Cancer/testis antigen 107, Homeobox protein from AL590526, PEPP subfamily gene 2, PEPP2, PEPP-2, Rhox homeobox family, member 2, Rhox homeobox family member 2, THG1, Testis homeobox gene 1, CT107

Gen-ID: 84528

NCBI Accession: NP_115887

UniProt: Q9BQY4