NKRF Antikörper (C-Term)
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- Target Alle NKRF Antikörper anzeigen
- NKRF (NFKB Repressing Factor (NKRF))
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Bindungsspezifität
- C-Term
- Reaktivität
- Human, Maus, Ratte, Hund, Pferd, Kaninchen, Rind (Kuh), Meerschweinchen, Schwein, Affe, Fledermaus, Hamster, Huhn
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NKRF Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human NKRF
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from C-Terminus of human NKRF (O15226, NP_060014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NKRF (NFKB Repressing Factor (NKRF))
- Andere Bezeichnung
- NKRF / NRF (NKRF Produkte)
- Hintergrund
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Name/Gene ID: NKRF
Synonyms: NKRF, NFkB-repressing factor, ITBA4, Protein ITBA4, NF-kappaB repressing factor, NFKB repressing factor, Transcription factor NRF, NF-kappa-B-repressing factor, NRF - Gen-ID
- 55922
- NCBI Accession
- NP_060014
- UniProt
- O15226
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