BCL11A Antikörper (N-Term)

Produktnummer ABIN6741578

Produktdetails anti-BCL11A Antikörper

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Schwein, Zebrafisch (Danio rerio), Huhn, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCL11A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human BCL11A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Chicken, Zebrafish (100%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human BCL11A (Q9H165, NP_075044). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Chicken, Platypus, Zebrafish (100%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu BCL11A

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Andere Bezeichnung: BCL11A (BCL11A Produkte)

Hintergrund: Name/Gene ID: BCL11A
Family: Cancer

Synonyms: BCL11A, B-cell CLL/lymphoma 11A, BCL11A-L, BCL11A-XL, BCL11a-M, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A-S, CTIP1, COUP-TF-interacting protein 1, HBFQTL5, EVI-9, KIAA1809, ZNF856, C2H2-type zinc finger protein, EVI9, Zinc finger protein 856

Gen-ID: 53335

NCBI Accession: NP_075044

UniProt: Q9H165

Pathways: Regulation of Cell Size