SOX17 Antikörper (C-Term)

Produktnummer ABIN6741583

Produktdetails anti-SOX17 Antikörper

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOX17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SOX17

Homologie: Percent identity by BLAST analysis: Human (100%) Guinea pig (92%) Mouse, Rat, Dog (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SOX17 (Q9H6I2, NP_071899). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Elephant, Panda, Bat, Pig, Guinea pig (92%), Galago, Marmoset, Mouse, Rat, Dog (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SOX17

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Andere Bezeichnung: SOX17 (SOX17 Produkte)

Synonyme: VUR3 antikoerper, SOX17 antikoerper, Sox17alpha antikoerper, Xsox17 antikoerper, Xsox17-alpha antikoerper, Xsox17alpha antikoerper, sox17-alpha antikoerper, tSox17alpha antikoerper, xSox17alpha1 antikoerper, xSox17alpha2 antikoerper, xsox17a antikoerper, sox17 antikoerper, sox7/17 antikoerper, Sox antikoerper, SRY-box 17 antikoerper, SRY (sex determining region Y)-box 17 antikoerper, SRY box 17 antikoerper, SRY-box 17 alpha antikoerper, sox7/17 protein antikoerper, SOX17 antikoerper, sox17 antikoerper, Sox17 antikoerper, sox17a antikoerper, sox7/17 antikoerper

Hintergrund: Name/Gene ID: SOX17
Family: Transcription factor

Synonyms: SOX17, Transcription factor SOX-17, VUR3

Gen-ID: 64321

NCBI Accession: NP_071899

UniProt: Q9H6I2