JAZF1 Antikörper (C-Term)

Produktnummer ABIN6741585

Produktdetails anti-JAZF1 Antikörper

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Zebrafisch (Danio rerio), Ratte, Meerschweinchen, Pferd, Kaninchen, Hund, Huhn, Rind (Kuh), Affe, Fledermaus, Schwein, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAZF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human JAZF1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human JAZF1 (Q86VZ6, NP_778231). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 7.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu JAZF1

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Andere Bezeichnung: JAZF1 (JAZF1 Produkte)

Synonyme: TIP27 antikoerper, ZNF802 antikoerper, JAZF1 antikoerper, AI591476 antikoerper, C820002C15 antikoerper, Jaz1 antikoerper, Tip27 antikoerper, zgc:103778 antikoerper, JAZF zinc finger 1 antikoerper, JAZF zinc finger 1b antikoerper, JAZF1 antikoerper, Jazf1 antikoerper, jazf1b antikoerper

Hintergrund: Name/Gene ID: JAZF1

Synonyms: JAZF1, ZNF802, TIP27, Zinc finger protein 802, JAZF zinc finger 1, TAK1-interacting protein 27

Gen-ID: 221895

NCBI Accession: NP_778231

UniProt: Q86VZ6