RPF1 Antikörper (AA 45-94)

Produktnummer ABIN6741590

Produktdetails anti-RPF1 Antikörper

Target: RPF1 (Brix Domain Containing 5 (RPF1))

Bindungsspezifität: AA 45-94

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Pferd, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human RPF1 / BXDC5

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine (100%) Dog, Armadillo (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa45-94 of human RPF1 (Q9H9Y2, NP_079341). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig (100%), Dog, Zebra finch, Chicken, Armadillo (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu RPF1

Target: RPF1 (Brix Domain Containing 5 (RPF1))

Andere Bezeichnung: BXDC5 / RPF1 (RPF1 Produkte)

Hintergrund: Name/Gene ID: RPF1

Synonyms: RPF1, BBR140, Brix domain containing 5, BXDC5, Ribosome production factor 1, RNA processing factor 1 (RPF1), RP11-118B23.1

Gen-ID: 80135

NCBI Accession: NP_079341

UniProt: Q9H9Y2