POLDIP3 Antikörper (AA 326-375)

Produktnummer ABIN6741591

Produktdetails anti-POLDIP3 Antikörper

Target: POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))

Bindungsspezifität: AA 326-375

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Meerschweinchen, Kaninchen, Affe, Fledermaus, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLDIP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human POLDIP3 / SKAR

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa326-375 of human POLDIP3 (Q9BY77, NP_115687). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus (85%), Sea squirt (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu POLDIP3

Target: POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))

Andere Bezeichnung: POLDIP3 / p46 (POLDIP3 Produkte)

Synonyme: PDIP46 antikoerper, SKAR antikoerper, 1110008P04Rik antikoerper, AA408269 antikoerper, AL022852 antikoerper, C77954 antikoerper, mKIAA1649 antikoerper, polymerase (DNA-directed), delta interacting protein 3 S homeolog antikoerper, DNA polymerase delta interacting protein 3 antikoerper, polymerase (DNA-directed), delta interacting protein 3 antikoerper, poldip3.S antikoerper, POLDIP3 antikoerper, Poldip3 antikoerper

Hintergrund: Name/Gene ID: POLDIP3

Synonyms: POLDIP3, p46, KIAA1649, SKAR, RNA-binding protein P46, PDIP46, S6K1 Aly/REF-like target

Gen-ID: 84271

NCBI Accession: NP_115687

UniProt: Q9BY77