PNPT1 Antikörper (AA 489-538)

Produktnummer ABIN6741592

Produktdetails anti-PNPT1 Antikörper

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Bindungsspezifität: AA 489-538

Reaktivität: Human, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNPT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PNPT1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PNPT1

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Andere Bezeichnung: PNPT1 (PNPT1 Produkte)

Synonyme: COXPD13 antikoerper, DFNB70 antikoerper, OLD35 antikoerper, PNPASE antikoerper, old-35 antikoerper, 1200003F12Rik antikoerper, Old35 antikoerper, PNPase antikoerper, Pnptl1 antikoerper, polyribonucleotide nucleotidyltransferase 1, mitochondrial antikoerper, polyribonucleotide nucleotidyltransferase 1 antikoerper, CpipJ_CPIJ005886 antikoerper, PNPT1 antikoerper, Pnpt1 antikoerper

Hintergrund: Name/Gene ID: PNPT1

Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1

Gen-ID: 87178

NCBI Accession: NP_149100

UniProt: Q8TCS8