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PNPT1 Antikörper (AA 489-538)

PNPT1 Reaktivität: Human, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Schwein WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6741592
  • Target Alle PNPT1 Antikörper anzeigen
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Bindungsspezifität
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 489-538
    Reaktivität
    • 41
    • 22
    • 18
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Schwein
    Wirt
    • 55
    • 3
    Kaninchen
    Klonalität
    • 57
    • 1
    Polyklonal
    Konjugat
    • 26
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PNPT1 Antikörper ist unkonjugiert
    Applikation
    • 49
    • 21
    • 13
    • 13
    • 10
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human PNPT1
    Homologie
    Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
    Top Product
    Discover our top product PNPT1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute with 50 μL sterile ddH2O.
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Andere Bezeichnung
    PNPT1 (PNPT1 Produkte)
    Synonyme
    COXPD13 antikoerper, DFNB70 antikoerper, OLD35 antikoerper, PNPASE antikoerper, old-35 antikoerper, 1200003F12Rik antikoerper, Old35 antikoerper, PNPase antikoerper, Pnptl1 antikoerper, polyribonucleotide nucleotidyltransferase 1, mitochondrial antikoerper, polyribonucleotide nucleotidyltransferase 1 antikoerper, CpipJ_CPIJ005886 antikoerper, PNPT1 antikoerper, Pnpt1 antikoerper
    Hintergrund
    Name/Gene ID: PNPT1

    Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1
    Gen-ID
    87178
    NCBI Accession
    NP_149100
    UniProt
    Q8TCS8
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