PODXL Antikörper (AA 301-350)

Produktnummer ABIN6741605

Produktdetails anti-PODXL Antikörper

Target: PODXL (Podocalyxin-Like (PODXL))

Bindungsspezifität: AA 301-350

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PODXL

Homologie: Percent identity by BLAST analysis: Human (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%), Gorilla (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: PODXL / Podocalyxin (PODXL Produkte)

Synonyme: Gp200 antikoerper, PC antikoerper, PCLP antikoerper, PCLP-1 antikoerper, PCB antikoerper, AW121214 antikoerper, Ly102 antikoerper, Pclp1 antikoerper, podocalyxin antikoerper, MEP21 antikoerper, PODXL antikoerper, Gp135 antikoerper, PCLP1 antikoerper, cPCLP1 antikoerper, podxl antikoerper, fc18d02 antikoerper, wu:fc18d02 antikoerper, Pc antikoerper, Pcb antikoerper, Podxl antikoerper, podocalyxin like antikoerper, pyruvate carboxylase antikoerper, podocalyxin-like antikoerper, PODXL antikoerper, PC antikoerper, Podxl antikoerper, podxl antikoerper, Pcx antikoerper

Hintergrund: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

Gen-ID: 5420

NCBI Accession: NP_001018121

UniProt: O00592

Pathways: Tube Formation