NR1I3 Antikörper (AA 180-229)
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- Target Alle NR1I3 Antikörper anzeigen
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
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Bindungsspezifität
- AA 180-229
- Reaktivität
- Human, Maus, Ratte, Hund, Meerschweinchen, Rind (Kuh), Schwein, Pferd, Kaninchen, Affe, Fledermaus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NR1I3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human NR1I3
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Bovine, Guinea pig (92%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%), Zebrafish (85%), Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product NR1I3 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
- Andere Bezeichnung
- NR1I3 / CAR (NR1I3 Produkte)
- Synonyme
- AA209988 antikoerper, AI551208 antikoerper, CAR antikoerper, CAR-beta antikoerper, Care2 antikoerper, ESTM32 antikoerper, MB67 antikoerper, CAR1 antikoerper, CXR antikoerper, nuclear receptor subfamily 1 group I member 3 antikoerper, nuclear receptor subfamily 1, group I, member 3 antikoerper, NR1I3 antikoerper, Nr1i3 antikoerper
- Hintergrund
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Name/Gene ID: NR1I3
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: NR1I3, Car-beta, CAR1, Constitutive active receptor, Constitutive active response, Orphan nuclear receptor MB67, MB67, CAR - Gen-ID
- 9970
- NCBI Accession
- NP_001070939
- UniProt
- Q14994
- Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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