YAP1 Antikörper (AA 351-400)

Produktnummer ABIN462247

Produktdetails anti-YAP1 Antikörper

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Bindungsspezifität: AA 351-400

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Affe, Hund, Schaf, Huhn, Rind (Kuh), Pferd, Schwein, Xenopus laevis, Kaninchen, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human YAP1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa351-400 of human YAP1 (P46937, NP_001123617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Andere Bezeichnung: YAP / YAP1 (YAP1 Produkte)

Synonyme: YAP antikoerper, YAP2 antikoerper, YAP65 antikoerper, YKI antikoerper, xyap antikoerper, yap antikoerper, yap2 antikoerper, yap65 antikoerper, yki antikoerper, AI325207 antikoerper, Yap antikoerper, Yap65 antikoerper, Yki antikoerper, Yorkie antikoerper, cb194 antikoerper, sb:cb194 antikoerper, si:ch211-181p1.5 antikoerper, si:dkey-3b8.3 antikoerper, wu:fc18c04 antikoerper, zgc:158380 antikoerper, Yes associated protein 1 antikoerper, Yes associated protein 1 S homeolog antikoerper, yes-associated protein 1 antikoerper, Yes-associated protein 1 antikoerper, YAP1 antikoerper, yap1.S antikoerper, Yap1 antikoerper, yap1 antikoerper

Hintergrund: Name/Gene ID: YAP1

Synonyms: YAP1, 65 kDa Yes-associated protein, YAP, Yes-associated protein beta, Yes-associated protein delta, Yorkie homolog, YAP2, Yes-associated protein 1, Yes-associated protein 2, YKI, YAP65

Gen-ID: 10413

NCBI Accession: NP_001123617

UniProt: P46937

Pathways: MAPK Signalweg, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha