MED4 Antikörper (AA 146-195)

Produktnummer ABIN6741609

Produktdetails anti-MED4 Antikörper

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Bindungsspezifität: AA 146-195

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Hund, Meerschweinchen, Pferd, Kaninchen, Huhn, Affe, Xenopus laevis, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MED4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human MED4 (Q9NPJ6, NP_054885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Pike, Zebrafish (100%), Drosophila, Mosquito (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Andere Bezeichnung: MED4 (MED4 Produkte)

Synonyme: zgc:110646 antikoerper, ARC36 antikoerper, DRIP36 antikoerper, HSPC126 antikoerper, TRAP36 antikoerper, VDRIP antikoerper, Vdrip antikoerper, 2410046H15Rik antikoerper, Vdirp antikoerper, mediator complex subunit 4 antikoerper, mediator complex subunit 4 S homeolog antikoerper, med4 antikoerper, med4.S antikoerper, MED4 antikoerper, Med4 antikoerper

Hintergrund: Name/Gene ID: MED4

Synonyms: MED4, ARC36, DRIP36, HSPC126, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36, TRAP36, VDRIP

Gen-ID: 29079

NCBI Accession: NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha